Habitat Suitability Evaluation of Polygala tenuifolia Based on MaxEnt Model and ArcGIS / 中国实验方剂学杂志

Objective:Polygalae Radix is mainly produced in Northeast，North，Northwest and Central China and Sichuan province. There are obvious differences in quality of <italic>Polygala tenuifolia</italic> from different areas. <italic>P. tenuifolia</italic> cultivation areas are relatively concentrated. Currently，there are only a few studies about the suitability zoning of <italic>P. tenuifolia</italic> nationwide，in order to determine the suitable zoning of <italic>P. tenuifolia</italic> in China. This paper made a habitat suitability evaluation of <italic>P. tenuifolia</italic> nationwide based on analysis of ecological factors in distribution areas of sampling points. Method:MaxEnt model was used to select ecological factors that affected the distribution of <italic>P. tenuifolia</italic> and construct an evaluation model. ArcGIS software was used to evaluate the suitability of different habitats of<italic> P. tenuifolia</italic>. The suitable areas were classified into high，medium，low suitability areas and unsuitable area of <italic>P. tenuifolia</italic> nationwide. Result:The evaluation model was highly accurate，and concluded that <italic>P. tenuifolia'</italic>s suitability distribution area in China was 3.21×10⁶ km²，including 0.52×10⁶ km² of high suitability area，0.96×10⁶ km² of medium suitability area and 1.73×10⁶ km² of low suitability area. High suitability areas were mainly distributed in Shanxi province，Hebei province，Shandong province，Shaanxi province，Liaoning province and Henan province. The main ecological factors affecting the distribution of <italic>P. tenuifolia</italic> were annual average temperature change range，mean temperature of driest quarter，precipitation of wettest quarter，altitude，slope and topsoil （0-30 cm） calcium carbonate. Conclusion:The results of the study are consistent with the records in relevant literatures，and can provide a theoretical basis for protection and cultivation of Chinese medicine resource <italic>P. tenuifolia</italic>.

Analysis on chemical constituents in Bupleuri Radix by HPLC-Q-TOF-MS / 中草药

Objective: To investigate the chemical constituents in extract of Bupleuri Radix using HPLC-Q-TOF-MS. Methods: The HPLC separation achieved on Agilent 1200 HPLC Diamonsil II C18 column (250 mm×4.6 mm, 5 μm) was used with a mobile phase of 0.05% H2O-formic acid (A) and acetonitrile (B), the flow rate was 1.0 mL/min, the column temperature was 35℃, and the detection wavelength was 200-600 nm. Positive and negative ions MS information and element analysis, via comparing MS data with those of the standard compounds and coupled with the related literature were used to analyze the main chemical constituents of BupleuriRadix. Results: Twenty-one chemical constituents were identified, including three phenolic acid, four flavonoids, and fourteen triterpenoid saponins, and isochlorogenic acid A (5), isochlorogenic acid B (6), 7,3'-di-O-methylquercetin (8), and 5-hydroxy-7-acetoxyflavone (24) were separated from Bupleuri Radix for the first time. Conclusion: It is used to quickly analyze the chemical constituents from Bupleuri Radix by establishing a rapid accurate evaluation method using HPLC-Q-TOF-MS, which could provide the references for the application development.